This B.G ADR ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.

The coated well immunoenzymatic assay for the quantitative measurement of serum ADR utilizes a monoclonal anti-ADR and a ADR-HRP conjugate. The assay sample and buffer are incubated together with anti-ADR antibody coated plate for sixty and washed. The diluted ADR-HRP conjugate is then added to each well and incubated. After the incubation period, the wells are decanted and washed three times. The wells are then incubated with a substrate for the enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stopping solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADR concentration since ADR from samples and ADR-HRP conjugate compete for the anti-ADR antibody binding site. Since the number of sites is limited, as more sites are occupied by ADR from the sample, fewer sites are left to bind ADR-HRP conjugate. Standards of known ADR concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of ADR. The unknown ADR concentration in each sample is interpolated from this curve.