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Alpha-Fetoprotein (AFP) ELISA Kit
Alpha-Fetoprotein (AFP) ELISA Kit

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The Alpha-Fetoprotein (AFP) ELISA is an enzyme-linked immunosorbent assay (ELISA) for the qualitative detection of Afp.

 

Advantage

 

1.Certificate:ISO13485:2003, GMP
2.Sensitivity>9/10
3.Specificity>14/15
4.Precision: CV<15%

 

 

Intended Use

 

The Alpha-Fetoprotein (AFP) ELISA is an enzyme-linked immunosorbent assay (ELISA) for the qualitative detection of Alpha fetoproteinin human serum or plasma.

 

KIT COMPONENTS

Microplate  1 plate (96 wells). Coated with Goat anti-AFP antibody. Reference standard set  6 vials (0.5ml/vial). Contains 0, 20, 50, 100, 200, 300ng/ml AFP, lyophilized. 1U=1IU=1.21ng.Control   2 vials (0.5ml/vial). Contains 40, 150ng/ml AFP.Conjugate  1 vial (12ml).  The solution contains HRP-labeled anti-AFP antibody, with Tris (6g/L) and stabilizing proteins, ready to use.Wash Buffer (25´)  1 vial (30ml).  Diluted 25-fold in distilled water as described in section of Preparation of Reagent. Substrate Solution A  1 vial (6ml).  Hydrogen peroxide-urea (approx. 0.6g/L) in citrate-acetate buffer solution (10 mmol/L).Substrate Solution B  1 vial (6ml).  Tetramethyl benzidine dihydrochloride (10g/L).Stop Solution 1 vial (6ml).   2N sulphuric acid.Plate Covers  2 pieces

 

SPECIFIC PERFORMANCE CHARACTERISTICS

Sensitivity

The minimum detectable concentration of AFP by this assay is estimated to be 10ng/ml. The sensitivity studies have been evaluated by using the national panel of purified Anti-AFP prepared by National Institute For the Control of Pharmaceutical and Biological Products (China), the minimal concentration of detection was not above 10mIU/ml.

Specificity

The cross-reaction values of CEA(1000ng/ml), CA125(600U/ml) and CA19-9 (500U/ml) with this kit were less than 10ng/ml.

Precision

The inner analysis precision: CV%<15.0; the inter analysis precision: CV%<20.0

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