one-step ELISA Kit for the rapid detection of nitrofurazolidone metabolite AOZ in animal tissues, fish and shrimp, honey, milk.

Nitrofurans are synthetic broad-spectrum antibiotics, which are frequently employed in animal production for its excellent antibacterial and pharmacokinetic properties. They had also been used as growth promoters in pig, poultry and aquatic production. In long term studies with lab animals indicated that the parent drugs and their metabolites showed carcinogenic and mutagenic characteristics. This has led to a prohibition of nitrofurans for the treatment of animals used for food production. The nitrofuran drugs furaltadone, nitrofurantoin and nitrofurazone were banned from use in food animal production in the EU in 1993, and the use of furazolidone was prohibited in 1995.

The analysis of nitrofuran drugs residue needs to be based on the detection of the tissue bound metabolites of the nitrofuran parent drugs. Since the parent drugs are very rapidly metabolized, and the tissue bound nitrofuran metabolites will retain for a long time, these metabolites are used as the target in the detection of the abuse of nitrofurans, which include Furazolidone metabolite (AOZ), Furaltadone metabolite (AMOZ), Nitrofurantoin metabolite (AHD) and Nitrofurazone metabolite (SEM).

AOZ-residues are determined most commonly by LC-MS or LC-MS/MS. Enzyme immunoassays, compared with chromatographic methods, show considerable advantages regarding sensitivity, detection limit, technical equipment and time requirement. (Time cost: 2h)