Quick Genomic DNA Purification from a Wide Range of Plant Sample Sizes
Contents and Storage
Kit Components | 50 preps | 200 preps |
Buffer FP1 | 25 ml | 100 ml |
Buffer FP2 | 10 ml | 40 ml |
Elution Buffer TE | 15 ml | 60 ml |
RNaseA (10 mg/ml) | 300 µl | 1.25 ml |
Store dry at room temperature (15-25°C).
Description:
DNAquick Plant Genomic DNA Kit use special buffer system for gDNA extraction from a wide range of fresh plant samples. Phenol extraction and ethanol precipitation are not required. DNAquick Plant Kits are available for different starting sample amounts by regulating the buffer volume. Purified DNA is suitable for a wide range of downstream applications.
Features:
Convenience: The procedure is quick and convenient in about 1 hour.
Safety: Phenol extraction and ethanol precipitation are not required.
Flexibility: No restriction to starting sample amount.
Applications:
Purified DNA is suitable for ------
PCR, real-time PCR and multiplex PCR
RADP, RFLP, AFLP and SSR
Library construction and Southern hybridization
Typical DNA yields:
Starting Sample | Sample Amount | Yield Range | OD260/OD280 |
Arabidopsis thaliana | 100 mg | 3-5 µg | 1.7-1.9 |
Wheat | 100 mg | 25-32 µg | 1.7-1.9 |
Pine needles | 100 mg | 25-32 µg | 1.7-1.9 |
Potato | 100 mg | 5-7 µg | 1.7-1.9 |
Tomato | 100 mg | 12-16 µg | 1.7-1.9 |
Cole | 100 mg | 2-5 µg | 1.7-1.9 |
Tobacco | 100 mg | 20-30 µg | 1.7-1.9 |
Rice | 100 mg | 12-30 µg | 1.7-1.9 |
Soy | 100 mg | 20-32 µg | 1.7-1.9 |
Corn | 100 mg | 20-32 µg | 1.7-1.9 |
* DNA yield depends on sample types; all data above gained from plant leaves.
