Pain pink red powder, free-flowing, homogeneous 5.0% solution, soluble in purified water upon boiling. Solution is pain yellow.
Identity Specifications
Dehydrated Appearance | Pain pink red powder, free-flowing, homogeneous |
Solution | 5.0% solution, soluble in purified water upon boiling. Solution is pain yellow. |
Prepared Appearance | Reddish purple, slightly opalescent |
Reaction of 5.0% Solution at 25C | pH 7.2 ± 0.4 |
Cultural Response
Prepare the medium per label directions. Inoculate and incubate at 35 ± 2C for 18-24 hours (and 40-48 hours for E. coli).
Escherichia coli | Partial inhibition, 2-3 zone growth，colony peach, with acid precipitation. |
Shigella bogdii Shigella sonnei Shigella flexneri | 1-3 zone growth, colony diameter≥1.6mm, colorless, opalescent. Circular. |
Enterobacter aerogenes | good growth, colorless opalescent circular colony. |
Salmonella sp Salmonella typhimurium | 1-4 zone growth, colony diameter1.7-2.0mm，opalescent circular. |
Staphylococcus aureu | Growth, pink colony |
Enterococcus faecalis | Partial inhibition |
Mixture culture | Streak inoculation of Escherichia coli and Salmonella typhimurium with ratio 1:1, typical Salmonella typhimurium can be seperated. |
Formulae
Approximate Formula* Per Liter
Peptone ....................................................... 17.0 g
Proteose Peptone ......................................... 3.0 g
Lactose ....................................................... 10.0 g
Sodium Chloride ............................................ 5.0 g
Agar ............................................................. 11.0 g
Neutral Red ............................................... 0.025 g
Bile Salts ....................................................... 5.0 g
Usage
Sepeartion of enterpathogen as well as enterococcal.
