... total bacteria , commonly used in aseptic filling empty PET bottle production line of sterility testing. Principle: peptone and yeast ... for 15minutes. 2. Inoculated the treated sample to LG medium, according to the specific requirements of sterility testing, vaccination ... appropriate sample size, the number of pieces of medium and positive control, the medium was placed in 28 ℃ ~ 32 ℃ prescribed ...