Quick Genomic DNA Purification from a Wide Range of Plant Sample Sizes with a Variety Starting Volume
Contents and Storage
Kit Components | 50 preps | 200 preps |
RNaseA (10 mg/ml) | 300 µl | 1.25 ml |
Buffer LP1 | 25 ml | 100 ml |
Buffer LP2 | 10 ml | 40 ml |
Buffer LP3 | 21 ml | 84 ml |
Buffer PW | 15 ml | 50 ml |
Elution Buffer TE | 15 ml | 60 ml |
TIANamp Spin Column CB3 | 50 | 200 |
Collection Tubes (2 ml) | 50 | 200 |
Store dry at room temperature (15-25°C).
Description:
DNAsecure new type Plant Genomic DNA Kit use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. DNAsecure Plant Genomic DNA Kit is immediately ready for use and can purify the Genomic DNA from a wide variety of plant species and tissues. Furthermore, phenol extraction and ethanol precipitation are not required.
Features:
Convenience: The procedure is quick and convenient in about 1 hour.
Safety: Phenol extraction and ethanol precipitation are not required.
High efficiency: 3-30 µg genomic DNA can be purified from 100 mg plant samples.
Applications:
High molecular weight DNA, up to 40 kb, can be isolation from plant samples using the Kit.
Typical DNA yields:
Starting Sample | Sample Amount | Yield Range | OD260/OD280 |
Arabidopsis thaliana | 100 mg | 3-4 µg | 1.7-1.9 |
Wheat | 100 mg | 25-30 µg | 1.7-1.9 |
Pine needles | 100 mg | 25-30 µg | 1.7-1.9 |
Potato | 100 mg | 4-6 µg | 1.7-1.9 |
Tomato | 100 mg | 10-15 µg | 1.7-1.9 |
Cole | 100 mg | 2-4 µg | 1.7-1.9 |
Tobacco | 100 mg | 20-25 µg | 1.7-1.9 |
Rice | 100 mg | 10-25 µg | 1.7-1.9 |
Soy | 100 mg | 20-30 µg | 1.7-1.9 |
Corn | 100 mg | 20-30 µg | 1.7-1.9 |
* DNA yield depends on sample types.
