The enzyme is used in enzymatic determination of K+, ATP, ADP, pyruvate and phophoenolpyruvate coupled with other enzymes.

This enzyme is purified from thermostable bacteria. It's useful in enzymatic determination of potassium, ATP, ADP, pyruvate and phophoenolpyruvate when coupled with  the related enzymes.

Reaction:
ATP + Pyruvate------ADP + phosphoenolpyruvate
Product Description
Appearance: White powder, lyophilized
Source: Microorganism
Enzyme Comission Number:EC 2.7.1.40
CAS Number: 9001-59-6
Storage Temperature -20C
Specific Activity:more than 300U/mg protein
Unit definition:One unit will convert one micromole of phosphoenolpyruvate to pyruvate
per min at pH 7.2 at 30C.
Preparation Instructions
The enzyme is reconstituted in 50mM Tris-HCl buffer, pH 8.5 for activity assay.
Properties
Molecular Weight: 68 kDa (SDS-PAGE)
Isoelectic point: 5.2
Michaelis constant: 1.1mM (ADP) 2.2mM(PEP)
Optimum pH: 7.5
Optimum temperature: 80C
pH Stability:5.0~10.0 (37C, 20hr)
Thermal Stability:< 60C (pH8.5, 20min)
Inhibitors:  Ag+,Hg2