It catalyzes the dehydrogenation of hydroxysteroid. It is useful for the determination of total bile acids in clinical analysis.

3alpha-Hydroxysteroid dehydrogenase catalyzes the dehydrogenation of hydroxysteroid. It is useful for the determination of total bile acids in clinical analysis.

Product Description
    Appearance: White powder, lyophilized
    Source: Microorganism
    Enzyme Comission Number:EC 1.1.1.50
    CAS Number: 9028-56-2
    Storage Temperature -20°C
    Specific activity: ≥ 50U/mg protein
    Unit definition:One unit will oxidate one micromole of androsterone per min at pH 8.9 at 25°C.

Properties
    Molecular Weight:28 kDa (SDS-PAGE)
    Isoelectic point: 7.5
    Michaelis constant: 3.0×10-5M(androsterone)     6.0×10-6M(NAD+)
    Optimum pH: 10.5
    Optimum temperature: 60°C
    pH Stability: 4.5~10.5
    Thermal Stability: < 50°C (pH7.2, 20min)
    Inhibitors: Ag+,Hg2+

 Preparation Instructions
    The enzyme is reconstituted in 30mM  potassium phosphate buffer, pH 7.2 for activity assay.