It convert oxaloacetate to malate, used for assay of L-malate and glutamate oxaloacetate transaminase (GOT) in clinical analysi

Malate dehydrogenase can convert oxaloacetate to malate. It’s useful for enzymatic determination of L-malate and  glutamate oxaloacetate transaminase (GOT) in clinical analysis.

 Product Description
    Appearance: White amorphous powder, lyophilized
    Source: Microorganism
    Enzyme Comission Number:EC 1.1.1.37
    CAS Number: 9001-64-3
    Storage Temperature -20°C
    Specific activity: ≥ 200U/mg protein
    Unit definition : One unit will cause the oxidation of one micromole NADH per min at pH 7.5 at 30°C.

Properties
    Molecular Weight:34 kDa (SDS-PAGE)
    Isoelectic point: 4.9
    Michaelis constant: 2.5×10-5M (NADH) 2.0×10-5M (Oxaloacetate)
    Optimum pH: 9.0
    Optimum temperature: 55°C
    pH Stability: 4.0~8.0
    Thermal Stability: < 60°C (pH7.5, 15min)
    Inhibitors: Ag+,Hg2+

Preparation Instructions
    The enzyme is reconstituted in 100mM potassium phosphate buffer, pH 7.5 containing 0.2% BSA for activity assay.